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Helicobacter Pylori Selective Agar
Used for selective isolation of Helicobacter pylori from clinical samples.
Catalog Number: AS-1249
Helicobacter pylori Selective Agar is a specialized culture medium designed for the isolation and cultivation of Helicobacter pylori. H. pylori is a bacterium associated with peptic ulcers and gastric cancer. In fact, the base medium is Columbia blood agar which must be supplemented with defibrinated sheep blood and a combination of antibiotics.
To ensure selective growth of H. pylori, the medium supplemented with a combination of antibiotics: vancomycin to inhibit Gram-positive bacteria, amphotericin B for antifungal activity, trimethoprim to suppress most enteric bacteria, and cefsulodin to inhibit Pseudomonas aeruginosa. These antibiotics allowing for the preferential growth of H. pylori.
The medium contains essential nutrients such as peptone and cornstarch to support bacterial growth. Sheep blood provides growth factors and facilitate hemolysis detection. Optimal growth conditions for H. pylori include a microaerophilic atmosphere and incubation at 42°C. Following isolation, biochemical tests are performed to confirm the identity of H. pylori.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Peptone, special | 23 |
| Corn starch | 1 |
| Sodium chloride | 5 |
| Agar | 15 |
| Final pH at 25°C | 7.3 ± 0.2 |
| Dehydrated Appearance | Beige, free-flowing, homogeneous. |
| Prepared Appearance with sheep blood | Cherry red, opaque, no hemolysis. |
| Reaction of 4.4% Solution at 25°C | pH 7.3 ± 0.2 |
| Incubate at 35 ± 2 °C for 18 to 48 hours. Keep the plates microaerophilic conditions (5-10% CO2). | ||
| Organism (ATCC) | Growth | Hemolysis |
| Helicobacter pylori (43526) | Good | None |
| Staphylococcus aureus (25923) | Inhibited | – |
| Candida albicans (10231) | Inhibited | – |
