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Tryptic Soy Broth, TSB
A general-purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms.
Catalog Number: AS-1371
Tryptic Soy Broth (TSB) is a complex liquid medium that is non-selective and is commonly used in microbiological quality control.
Peptones provide the main source of carbon and nitrogen, dextrose for the metabolism of carbohydrates, and dibasic potassium phosphate as a buffer, TSB offers a well-balanced nutritional condition for the growth of a variety of microorganisms. Sodium chloride maintains the medium equilibrium.
Pharmacopoeias and international standards for microbial enumeration and sterility testing accept TSB as a standard medium.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Pancreatic digest of Casein | 15 |
| Papaic digest of Soybean Meal | 5 |
| Sodium Chloride | 5 |
| Dipotassium Hydrogen Phosphate | 2.5 |
| Dextrose | 2.5 |
| Final pH at 25°C | 7.3 ± 0.2 |
| Dehydrated Appearance | Light beige, free-flowing, homogeneous. |
| Prepared Appearance | Light amber, clear. |
| Reaction of 3.0% Solution at 25°C | pH 7.3 ± 0.2 |
| Incubate at 30 to 35 °C for 18 to 72 hours. | |
| Organism (ATCC) | Recovery |
| Escherichia coli (8739) | Good |
| Bacillus subtilis (6633) | Good |
| Streptococcus pneumoniae (6305) | Good |
| Candida albicans (10231) | Good |
| Pseudomonas aeruginosa (9027) | Good |
