Brilliant-green Bile Broth 2%, BGBB

For selectively confirming the presence of coliform bacteria in water, wastewater, food, and dairy products, Brilliant Green Bile Broth 2% (BGBB) is utilized. After a test for suspected coliform, BGBB distinguishes and verifies the existence of these microorganisms. Key ingredients that determine its selectivity are lactose, a fermentable sugar that coliforms needed to produce gas, peptone, a source of vital nutrients, brilliant green, and ox bile. The last two function as selective agents, inhibiting the majority of Gram-positive and many Gram-negative bacteria. This avoids misleading bacteria like the lactose-fermenting anaerobe Clostridium perfringens from causing false positive results. The generation of gas in BGBB, which is usually detected at 30°C or 37°C because of lactose fermentation, verifies the presence of coliforms, which are an essential sign of possible fecal contamination and deteriorated food or water quality.
BGBB is suggested by APHA and ISO standards for coliform confirmation.

Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.