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APT Broth
Used for cultivating heterofermentative lactic acid bacteria with high thiamine content requirement.
Catalog Number: AS-1121
APT (All-purpose Tween 80) Broth is a nutrient-rich medium used to cultivate lactic acid bacteria like Lactobacillus viridescens ATCC 12706, which is necessary for thiamine microbiological assays.
APT Broth’s composition is like APT Agar. The American Public Health Association (APHA) recommends it for microbiological examination of lactic acid bacteria in meat, dairy products, and vegetable.
APT Broth includes yeast extract, dextrose, polysorbate 80, and casein enzymic hydrolysate to supports the growth of a wide variety of microorganisms, including potential contaminations like coliform bacteria, even though it was originally intended for these fastidious microorganisms. For lactic acid bacteria to grow, metallic salts must be present.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Casein enzymic hydrolysate | 12.5 |
| Yeast extract | 7.5 |
| Dextrose | 10 |
| Sodium citrate | 5 |
| Sodium chloride | 5 |
| Dipotassium phosphate | 5 |
| Magnesium sulphate | 0.8 |
| Manganese chloride | 0.14 |
| Ferrous sulphate | 0.04 |
| Polysorbate 80 | 0.2 |
| Thiamine hydrochloride | 0.001 |
| Final pH at 25°C | 6.7 ± 0.2 |
| Dehydrated Appearance | Cream to yellow, free-flowing, homogeneous. |
| Prepared Appearance | Yellow, Clear solution. |
| Reaction of 4.62% Solution at 25°C | pH 6.7 ± 0.2 |
| Incubate at 35±2 °C for 18 to 24 hours. | |
| Organism (ATCC) | Recovery |
| Lactobacillus acidophilus (4356) | Good |
| Lactobacillus viridescens (12706) | Good |
| Leuconostoc mesenteroides (12291) | Good |
| Lactobacillus casei (9595) | Good |
| Lactobacillus plantarum (8014) | Good |
