Brilliant-green Phenol-red Lactose Sucrose (BPLS) Agar

Brilliant Green Agar (BGA), sometimes referred to as BPLS agar, is a selective culture medium that is mostly used for Salmonella species isolation. Brilliant green, which inhibits the growth of most Gram-positive and many Gram-negative bacteria, is thought to be responsible for its selective properties. BPLS is frequently used in tandem with less inhibitory enrichment broths, such as selenite or tetrathionate, to improve recovery rates. Peptones supply vital nutrients, lactose and sucrose offer energy from carbohydrates, phenol red indicates pH, and sodium chloride preserves osmotic balance are the components of the medium.
Bacteria that do not ferment lactose or sucrose, like Salmonella, usually form pink to red colonies on BPLS agar. Conversely, because lactose and/or sucrose fermenters produce acid, their colonies appear yellow to green. Even though BPLS is quite selective, to ensure proper identification of suspected Salmonella isolates, confirmation testing must be carried out.

Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.