Buffered Peptone Water, BPW

A non-selective pre-enrichment medium called Buffered Peptone Water (BPW) was created expressly to identify and enumerate Salmonella in food and water samples. Widely accepted and defined by multiple ISO protocols (ISO 11133, ISO 11290, ISO 19250, ISO 21528, ISO 6579, ISO 6887), BPW has a crucial role in the recovery of Salmonella cells that have been slightly harmed, often caused by conditions related to food processing.
Peptone, sodium chloride, and a phosphate buffer system, among other nutrient-rich ingredients in BPW, create ideal circumstances for bacterial growth and resuscitation. The medium lessens the harmful effects of acidic environments on injured cells by preserving a constant pH. Pre-enrichment in BPW is important for amplifying the target organism prior to selective enrichment processes because Salmonella is generally not present in food matrices.
In addition to Salmonella, BPW is also used in the counting of Listeria monocytogenes and as a diluent for other microbes. Samples are routinely inoculated into BPW and then allowed to incubate to facilitate bacterial repair and growth. The enhanced culture is then moved to selective media so that Salmonella can be isolated and identified using distinctive colony morphology.

Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.