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Giolitti-Cantoni Broth Base
For enriching Staphylococcus aureus from meals during isolation procedures.
Catalog Number: AS-1238
Giolitti-Cantoni A selective enrichment medium, created especially to identify Staphylococcus aureus in food materials. It has a nutrient-rich basis of peptone, yeast extract, beef extract, mannitol, and sodium pyruvate to stimulate staphylococcal growth. It is recommended by authoritative authorities like the ISO, APHA, and IDF. Lithium chloride, which inhibits Gram-negative bacteria, and potassium tellurite and glycine, which suppress Gram-positive bacteria, are thought to be responsible for the medium’s selective qualities. To prevent micrococcal growth, sterile paraffin wax is used, which produces anaerobic conditions. The medium turning black suggests possible Staphylococcus aureus infection. The purpose of this broth is to qualitatively identify the presence of low staphylococci in food samples.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Tryptone | 10 |
| Beef Extract | 5 |
| Yeast Extract | 5 |
| D-Mannitol | 20 |
| Sodium Chloride | 5 |
| Lithium Chloride | 5 |
| Sodium Pyruvate | 3 |
| Tween 80 | 1 |
| Final pH at 25°C | 6.9 ± 0.2 |
| Dehydrated Appearance | Tan, free-flowing, homogeneous. |
| Prepared Appearance | Medium amber, clear. |
| Reaction of 5.52% Solution at 25°C | pH 6.9 ± 0.2 |
| Incubate at 35±2 °C for 24 to 48 hours. | ||
| Organism (ATCC) | Recovery | Appearance |
| Escherichia coli (25923) | Inhibition | No Blackening |
| Staphylococcus aureus (6538) | Good | Black |
| Staphylococcus aureus (25923) | Good | Black |
