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Lactose Broth
Used to detect the presence of coliform organisms, as a pre-enrichment broth for salmonella and in general, for the study of lactose fermentation bacteria.
Catalog Number: AS-1263
Lactose broth is a non-selective enrichment medium. The main application of lactose broth is to presume detection of coliform bacteria in food, dairy, and water. Peptone and beef extract are included as sources of nutrients, and lactose is the main carbohydrate used in fermentation. Durham tubes contain gas that is produced when lactose is fermented by coliform bacteria. This medium is frequently used as a pre-enrichment stage for the isolation of Salmonella and is advised by the APHA for the detection of coliforms. Double-strength Lactose Broth may be required for large sample volumes to retain the proper concentrations of nutrients.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | (gr/L) |
| Beef extract | 3 |
| Peptone | 4 |
| Lactose | 5 |
| Final pH at 25°C | 6.9 ± 0.2 |
| Dehydrated Appearance | Light beige to light tan, free flowing, homogeneous. |
| Prepared Appearance | Light amber, clear. |
| Reaction of 1.3% Solution at 25°C | pH 6.9 ± 0.2 |
| Incubate at 35±2 °C for 18 to 48 hours. | ||
| Organism (ATCC) | Recovery | Gas |
| Escherichia coli (25922) | Good | + |
| Enterobacter aerogenes (13048) | Good | + |
| Enterococcus faecalis (19433) | Good | – |
| Salmonella enterica (6539) | Good | – |
