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LB Broth, Miller
Used for cultivation of E. coli in fermentation and molecular genetic studies.
Catalog Number: AS-1270
Luria-Bertani (LB) Broth, Miller modification is a nutrient-rich medium used especially for the support and cultivation of Escherichia coli strains, especially recombinant variants. LB broth, which is derived from the original Lennox medium, has a greater sodium chloride concentration to promote osmotic balance and membrane transfer. The growth of E. Coli K12 strains, which frequently have auxotrophic needs, is optimized with this mixture. Essential amino acids, peptides, vitamins, and other growth factors are provided by tryptone and yeast extract. To meet the needs of a particular strain, the medium can be further supplied with glucose and have the salt concentrations changed.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Tryptone | 10 |
| Yeast Extract | 5 |
| Sodium Chloride | 10 |
| Final pH at 25°C | 7.0 ± 0.2 |
| Dehydrated Appearance | Tan, free-flowing, homogeneous. |
| Prepared Appearance | Light amber, very slightly to slightly opalescent. |
| Reaction of 2.5% Solution at 25°C | pH 7.0 ± 0.2 |
| Incubate at 35±2 °C for 18 to 48 hours. | |
| Organism (ATCC) | Recovery |
| Escherichia coli (25922) | Good |
| Enterococcus faecalis (19433) | Good |
| Pseudomonas aeruginosa (27853) | Good |
