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LMX Broth, Modified
Used for simultaneous detection of total coliforms and E. coli in water, food and dairy products by the fluorogenic procedure.
Catalog Number: AS-1277
LMX Broth, modified, was created to quickly identify and distinguish between E. coli and total coliforms. Manafi and Ossmer improved upon the original formulation of Kneifel and Manafi. Phosphate buffers promote the growth of coliform bacteria, whereas lauryl sulfate inhibits Gram-positive bacteria. The simultaneous detection of E. coli and total coliforms is made easier by the addition of chromogenic and fluorogenic substrates. Coliforms are indicated by a color shift from yellow to blue-green, and E. coli is confirmed by blue fluorescence when exposed to UV light. Enzyme activity is increased by tryptophan and 1-isopropyl-β-D-1-thio-galactopyranoside, which also raises the sensitivity and specificity of the medium.
Storage
Store dehydrated medium and prepared medium at 2-8 °C.
| Composition | gr/L |
| Tryptose | 5 |
| Sodium Chloride | 5 |
| Sorbitol | 1 |
| Tryptophan | 1 |
| Dipotassium hydrogen phosphate | 2.7 |
| Potassium dihydrogen phosphate | 2 |
| Lauryl sulfate sodium salt | 0.1 |
| X-GAL | 0.08 |
| MUG | 0.05 |
| IPTG | 0.1 |
| Final pH at 25°C | 6.8 ± 0.2 |
| Dehydrated Appearance | Cream to light yellow, homogeneous, free flowing. |
| Prepared Appearance | Cream to light yellow. |
| Reaction of 1.17% Solution at 25°C | pH 6.8 ± 0.2 |
| Incubate at 35 ± 0.5 °C for 18 to 48 hours. | |||
| Organism (ATCC) | Color Change to Blue-Green | Fluorescence | Indole |
| Escherichia coli (25922) | + | + | + |
| Shigella flexneri (12022) | – | – | – |
| Enterobacter aerogenes (13048) | + | – | – |
| Salmonella typhimurium (14028) | – | – | – |
| Citrobacter freundii (8090) | + | – | – |
