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Malt Extract Agar
For detection, isolation and enumeration of fungi, particularly yeasts and molds.
Catalog Number: AS-1286
The identification of fungi in laboratories is mostly carried out by direct methods, especially with the use of malt and malt extracts for the growth of mold and yeast.
Reddish first suggested a malt extract-based growth medium as a good substitute for yeast. This Malt Extract Medium is used to identify, isolate, and count yeasts and molds. It is comparable to the formula developed by Galloway and Burgess.
Malt extract provides nutrients that is suitable to the growth of mold and yeast. Peptone supports strong growth with particular morphology and pigmentation. For accurate mycological counts and to prevent bacterial growth, add 10% lactic acid to the medium.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Malt Extract | 30 |
| Mycological Peptone | 5 |
| Agar | 15 |
| Final pH at 25°C | 5.4 ± 0.2 |
| Dehydrated Appearance | Light tan, free-flowing, homogeneous. |
| Prepared Appearance | Light to medium amber, very slightly to slightly opalescent. |
| Reaction of 4.8% Solution at 25°C | pH 5.4 ± 0.2 |
| For 18 to 48 hours, incubate at 30 ± 2°C. | |
| Organism (ATCC) | Recovery |
| Candida albicans (10231) | Good |
| Aspergillus brasiliensis (niger) (16404) | Good |
| Saccharomyces cerevisiae (9763) | Good |
