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Nutrient Broth
General culture media for cultivating less fastidious microorganisms.
Catalog Number: AS-1309
Nutrient broth is a common non-selective culture medium in microbiology. It supports the growth of a wide range of non-fastidious bacteria. Peptone and yeast extract supplies vital amino acids, peptides, vitamins, and minerals required for the growth of microorganisms. Sodium chloride preserves the osmotic equilibrium of the medium.
Nutrient broth is used in many different microbiological processes, including enrichment, culture maintenance, and purity evaluation before further biochemical or immunological testing.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Peptone | 5 |
| Yeast Extract | 2 |
| Beef Extract | 1 |
| Sodium Chloride | 5 |
| Final pH at 25°C | 7.4 ± 0.2 |
| Dehydrated Appearance | Medium tan, free-flowing, homogeneous. |
| Prepared Appearance | Light to medium amber, clear. |
| Reaction of 1.3% Solution at 25°C | pH 7.4 ± 0.2 |
| incubate at 35 ± 2 °C for 18 to 24 hours. | |
| Organism (ATCC) | Recovery |
| Escherichia coli (25922) | Good |
| Enterococcus faecalis (19433) | Good |
| Staphylococcus aureus (25923) | Good |
