Safranin O Powder, a common biological stain, is widely used in microbiology and histology for staining purposes.
Microbiologists employ Safranin O Powder to stain bacterial cells, facilitating the differentiation of Gram-positive and Gram-negative bacteria based on their cell wall characteristics. Gram-positive bacteria retain the stain, appearing purple, while Gram-negative bacteria do not, appearing red or pink post-counterstaining.
In histology, Safranin O Powder is crucial for staining tissues, especially for highlighting cell nuclei and cartilage in histological samples. This staining process enables researchers and pathologists to visualize cellular structures and study tissue architecture.
Beyond microbiology and histology, Safranin O Powder finds application in dyeing textiles and leather, showcasing its versatility beyond scientific research.
In summary, Safranin O Powder is pivotal in microbiology and histology labs. Its use in staining bacterial cells, highlighting tissue structures, and its versatility in other industries make it an indispensable dye in scientific research and various applications.
Cat. Number | ASC-1011 |
CAS Number | 477-73-6 |
MDL Number | MFCD00011759 |
Color Index | 50240 |
Pubchem | 310279833 |
Molecular Weight | 350.85 gr/mol |
Molecular Formula | C20H19ClN4 |
Storage Temperature | 20 °C / Keep away from oxidizing agents. |
Form and Color | Crystall / Red-brown to dark brown |
Purity | ≥ 95 % |
Solution Appearance | 0.1% in 50% EtOH: Red to dark red, clear solution |
Solubility | Soluble in water 50 gr/L Soluble in alcohol |
Wavelength of Maximum Absorption (λ max) | 530 - 534 nm (in 50% EtOH) |
Specific Absorption | ≥ 1500 (λ max, in 50% EtOH) |
Loss on Drying | ≤ 8 % |
Synonym | Basic Red2 / Cotton Red / Gossypimine / Safranin Y or A / 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride / Safranine T |