TMPPD Powder, also known as N, N, N”, N” – Tetramethyl-p-phenylenediamine Dihydrochloride, plays a crucial role as an oxidizable compound in scientific research. It serves as a valuable reducing substrate for heme peroxidases, facilitating various biochemical assays. This compound is particularly useful in enzyme detection and microbial identification in microbiology.
In the realm of heme peroxidase reactions, TMPPD Powder acts as a critical component. It serves as a reducing agent, aiding in the conversion of substrates by heme peroxidases. This functionality is vital for studying enzyme kinetics and understanding biological processes involving peroxidases.
Additionally, microbiologists widely employ TMPPD Powder as a test reagent. It helps determine the presence of cytochrome oxidase-positive microorganisms. This method is crucial for distinguishing microbial species based on their oxidase activity, contributing to microbial classification and identification.
Another significant application of TMPPD Powder is in detecting peroxidases on polyacrylamide gels. Its interaction with peroxidases leads to visible reactions, making it a valuable tool for enzyme detection and analysis.
In summary, TMPPD Powder is an essential component in microbiology and biochemical research. Its roles in heme peroxidase reactions, microbial identification, and enzyme detection highlight its versatility and importance in scientific studies. Whether used as a reducing substrate or a test reagent, TMPPD Powder continues to be a valuable asset in laboratories worldwide, aiding researchers in their quest to understand biochemical and microbial processes.
Cat. Number | ASC-1010 |
CAS Number | 637-01-4 |
MDL Number | MFCD00012482 |
Pubchem | 310280816 |
Molecular Weight | 237.17 gr/mol |
Molecular Formula | C10H16N2 . 2HCl |
Storage Temperature / Condition | 20°C / Keep away form light and strong oxidizing agents |
Form and Color | Powder or solid / White to grey or brown |
Solution Appearance (5 % in water) | Clear to slightly turbid solution |
Solubility | Soluble in Dimethyl sulfoxide (DMSO) / Water / Phosphate buffered saline (pH 7.2) |
Assay | ≥ 97 % |
Synonym | TMPPD / Wursters reagent / TMPD hydrochloride / 1,4-Bis(dimethylamino)benzene dihydrochloride / wurster’s blue dihydrochloride |