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2,3,5-Triphenyltetrazolium chloride, TTC

Enzyme Substrate for Dehydrogenase

Catalog Number: ASC-1021
CAS Number: 298-96-4
MW: 334.80 g/mol
MF: C19H15ClN4

The dye 2,3,5-triphenyltetrazolium chloride (TTC) is primarily utilized for counting microbial colonies in solid culture media. When oxidized, this dye is colorless; when reduced by microbes by dehydrogenase enzyme, formazan is formed, which turns the dye red. Via enzymatic action, live microorganisms break down TTC, generating red formazan that is stored in cell granules. Dead cells stay colorless, whereas living cells turn red.

Cat. NumberASC-1021
CAS Number298-96-4
MDL NumberMFCD00011963
PubChem310281422
Molecular Weight334.80 g/mol
Molecular FormulaC19H15ClN4
Storage Temperature+4°C
Form and ColorWhite to faint yellow, Powder
Solubility (5% in ethanol)Clear, Faint yellow to yellow solution
Melting Point238 – 243 °C
Loss on Drying≤ 3.0% (120°C, 3h)
Assay≥ 99.0% (dried basis)
SynonymTT, TPTZ, TTZ, Tetrazolium Red

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