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APT Agar
Used for cultivating heterofermentative lactobacilli and other organisms with high thiamine content requirement.
Catalog Number: AS-1120
A non-selective culture medium called APT (All-Purpose Tween 80) Agar is intended to enhance the growth of lactic acid bacteria of all kinds, especially heterofermentative species like Lactobacillus, Leuconostoc, and Lactococcus. Peptone, yeast extract, glucose, and Tween 80 are added to the medium as major nutrients, in addition to necessary mineral salts. Fatty acids are included in Tween 80 and are essential for lactobacilli’s metabolism. These bacteria are isolated and enumerated using APT Agar from a variety of food matters like meat, dairy, and fruit juices. The medium’s ability to enhance the growth of organisms that require thiamine also makes it suitable for use in thiamine microbiological assays.
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.
| Composition | gr/L |
| Peptone from Casein | 12.5 |
| Yeast Extract | 7.5 |
| D (+) glucose | 10 |
| Sodium Chloride | 5 |
| Tri-sodium Citrate | 5 |
| Dipotassium Hydrogen Phosphate | 5 |
| Tween 80 | 0.2 |
| Magnesium Sulfate | 0.8 |
| Manganese Chloride | 0.14 |
| Iron (II) Sulfate | |
| 0.04 | 0.04 |
| Thiamine Dichloride | 0.001 |
| Agar | 13.5 |
| Final pH at 25°C | 6.7 ± 0.2 |
| Dehydrated Appearance | Light beige, free-flowing, homogeneous. |
| Prepared Appearance | Medium amber, clear to slightly opalescent, may have a slight precipitate. |
| Reaction of 5.97% Solution at 25°C | pH 6.7 ± 0.2 |
| Incubate at 35±2 °C for 24 to 48 hours. | |
| Organism (ATCC) | Recovery |
| Lactobacillus fermentum (9338) | Good |
| Lactobacillus acidophilus (4356) | Good |
| Weissella viridescens (12706) | Good |
