The bile salts mixture plays a crucial role in microbiology, in microbial culture media acting as a selective inhibitor against gram-positive bacteria while facilitating the growth of bile-tolerant enteric microorganisms. Bile salts mixture Typically used at concentrations of 0.25–0.5%, this mixture serves a dual purpose by inducing spore germination while inhibiting outgrowth, ensuring accurate microbial testing results.
The Bile Salts Mixture is used in different culture media, enhancing their selectivity and specificity. It does not affect the color of pH indicator dyes in prepared media and after bacterial culture. Here are some important points about the bile salts mixture:
- Acts as a selective inhibitor against gram-positive bacteria.
- Facilitates the growth of bile-tolerant enteric microorganisms.
- Induces spore germination while inhibiting outgrowth.
- Used at concentrations of 0.25–0.5% in various culture media.
- Enhances the selectivity of culture media to Enterobacteriaceae.
- Helps detect lactose-fermenting bacteria by causing precipitation around acid-producing colonies.
In summary, the bile salts mixture is a valuable tool in microbiological laboratories, ensuring accurate and reliable results in microbial testing. Its dual action of inhibiting unwanted bacteria while promoting the growth of target microorganisms makes it an essential component in microbial culture media.
Composition
| Bile salts | ≥45 % |
| Moisture | ≤6 % |
Shelf life and storage
Store the bile salts mixture container between 10-30 °C in a ventilated area and protected from light. Close the container tightly after use. Use before expiry date.
Note that this product is for R&D use only. DO NOT USE for drug, household, or any other uses.
