IPTG Powder, also known as Isopropyl β-D-1-thiogalactopyranoside, acts as an allolactose analogue that remains unmetabolized within cells. It plays a key role in molecular biology, inducing the overexpression of recombinant proteins controlled by the lac promoter in Escherichia coli.
When introduced to bacterial cultures, IPTG binds to the lac repressor protein, blocking its inhibition of gene expression. This allows for precise control over the production of large quantities of the desired protein.
Researchers widely use IPTG Powder in the laboratory to induce protein expression in bacterial cultures, aiding in the study of protein functions, structures, and interactions. Additionally, IPTG is pivotal in recombinant DNA technology, assisting scientists in cloning and expressing genes of interest.
IPTG’s significance extends to protein purification processes, where its role in inducing the expression of target proteins allows for their isolation and purification for further analysis.
In summary, IPTG Powder’s function as an inducer of gene expression, especially in the production of recombinant proteins, is vital in molecular biology and biotechnology. Its precise regulation of protein expression and assistance in protein purification processes make it an invaluable tool for researchers in various scientific fields.
| Cat. Number | ASC-1017 |
| CAS Number | 367-93-1 |
| MDL Number | MFCD00063273 |
| Pubchem | 310275355 |
| Molecular Weight | 238.30 gr/mol |
| Molecular Formula | C9H18O5S |
| Storage Temperature | +4 °C |
| Form and Color | Crystalline powder / White |
| Solubility | Soluble in Water and methanol |
| Purity (HPLC) | ≥ 98% |
| UV Absorbance (5% in water) | 300 nm: ≤ 0.15 / 400 nm: ≤0.06 |
| pH (5% in water) | 5 – 7 |
| Melting Point | 110 – 114 °C |
| Water Content | ≤ 1 % |
| Synonym | Isopropyl-beta-D-thiogalactopyranoside, approx. 15.0% dioxane |
