Bromocresol Green Powder, a widely used pH indicator, plays a crucial role in laboratories for various applications.
Microbiologists utilize Bromocresol Green Powder to determine the pH of microbiological media. Its color change from yellow to blue assists in assessing the acidity or alkalinity of the media, promoting the growth of specific bacterial strains.
Moreover, in biochemical assays, researchers monitor reactions based on pH changes using Bromocresol Green Powder. Its transition from yellow to blue serves as a visual cue for reaction progress and completion.
Furthermore, researchers rely on Bromocresol Green Powder in studies of enzyme kinetics. By monitoring color changes in the presence of enzymes, valuable insights into enzyme activity and reaction rates are gained.
In clinical laboratories, Bromocresol Green Powder aids in analyzing serum protein levels. It facilitates the quantitative determination of albumin in blood serum, assisting in the diagnosis of various medical conditions.
Additionally, this dye is essential for DNA tracking in agarose gel electrophoresis, binding to proteins and enabling the detection of proteins with an optical sensor.
Furthermore, Bromocresol Green Powder can reversibly react with hydrochloric acid in a pH-dependent manner. If this dye binds to serum proteins, a decrease in fluorescent intensity occurs.
In summary, Bromocresol Green Powder’s role as a pH indicator extends to microbiology, biochemistry, enzyme kinetics, and clinical diagnostics. Its versatility, stability, and ease of use make it a valuable asset in laboratory settings across different fields of study.
| Cat. Number | ASC-1012 |
| CAS Number | 76-60-8 |
| MDL Number | MFCD00005874 |
| Pubchem | 310268866 |
| Molecular Weight | 698.02 gr/mol |
| Molecular Formula | C21H14Br4O5S |
| Storage Temperature / Condition | 20 °C / Keep away from strong oxidizing agents. |
| Form and Color | Powder / Light yellow to orange to brown |
| Solution Appearance (0.1 % in ethanol) | Clear |
| Solubility | Soluble in Benzene, ethanol, and diethyl ether. Partially soluble in water. |
| Absorptivity (1% | 1cm), (pH 5.4, at max 616 nm) | 530 to 570 |
| Absorptivity (1% | 1cm), (pH 3.8, at max 443 nm) | 240 to 270 |
| pH Transition Range | 3.8 (Yellow-Green) – 5.4 (Blue) |
| Loss on Drying | ≤ 3 % |
| Synonym | 3’, 3”, 5’, 5” – Tetrabromo-m-cresolsulfonephthalein; Bromocresol Green Sultone form |
