Bromocresol Purple Powder, also known as Bromocresol Purple, serves as a versatile biological stain and pH indicator in microbial culture media. Its distinct color shift from yellow to purple aids in monitoring pH changes, crucial for optimizing bacterial growth conditions.
In microbiology, Bromocresol Purple Powder’s pH indicator property allows for precise adjustments to culture media, ensuring optimal conditions for bacterial growth.
Additionally, it exhibits colistin resistance by binding to lipopolysaccharides, hindering colistin’s effectiveness. This property is valuable for studying mechanisms of colistin resistance in bacteria.
Furthermore, Bromocresol Purple Powder finds application in clinical labs to measure serum albumin levels. Its color change in response to protein binding enables accurate quantification of albumin in blood serum, aiding in medical diagnosis and monitoring.
In summary, Bromocresol Purple Powder’s role as a pH indicator, colistin resistance, serum albumin quantification, and environmental analysis applications highlight its multifaceted importance in various scientific fields.
Cat. Number | ASC-1013 | |||
CAS Number | 115-40-2 | |||
MDL Number | MFCD00011681 | |||
Pubchem | 310268868 | |||
Molecular Weight | 540.24 gr/mol | |||
Molecular Formula | C21H16Br2O5S | |||
Storage Temperature | 20 °C | |||
Form and Color | Powder / Grey to purple, pink or yellow-brown | |||
Solution Appearance (0.1 % in ethanol) | Clear, Yellow | |||
Solubility | Soluble in ethanol (80 mg/ml), alcohol, methanol (4 mg/ml), methyl cellosolve (300 mg/ml), dilute alkalies. Insoluble in water. | |||
Purity | ≥ 95 % | |||
pH Transition Range | 5.2 (Greenish-yellow) – 6.8 (Purple-blue) | |||
Wavelength of Maximum Absorption | λ max1: ̴ 431.0nm / λ max2: ̴ 589.0nm | |||
Absorptivity (1%/1cm) | 400 - 450 (pH 5.2 at λ max1) / 1000 - 1100 (pH 6.8 at λ max2) | |||
Synonym | 5,5’-Dibromo-o-cresolsulfonphthalein; Bromocresol Purple sultone form |