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Bromocresol Green Powder
Bromocresol Green Powder’s role as a pH indicator extends to microbiology, biochemistry, enzyme kinetics, and clinical diagnostics.
Catalog Number: ASC-1012
CAS Number: 76-60-8
MW: 698.02 gr/mol
MF: C21H14Br4O5S
Bromocresol Green is a biological stain used in microbial culture media as pH indicator. Moreover, DNA tracking in agarose gel electrophoresis uses this color. Bromocresol green binds to proteins, so, it can be used for detecting proteins with an optical sensor.
Bromocresol green can reversibly react with hydrochloride acid in a pH dependent manner. Note that if Bromocresol Green binds to serum proteins, a decrease in fluorescent intensity will happen.
| Cat. Number | ASC-1012 |
| CAS Number | 76-60-8 |
| MDL Number | MFCD00005874 |
| Pubchem | 310268866 |
| Molecular Weight | 698.02 gr/mol |
| Molecular Formula | C21H14Br4O5S |
| Storage Temperature / Condition | 20 °C / Keep away from strong oxidizing agents. |
| Form and Color | Powder / Light yellow to orange to brown |
| Solution Appearance (0.1 % in ethanol) | Clear |
| Solubility | Soluble in Benzene, ethanol, and diethyl ether. Partially soluble in water. |
| Absorptivity (1% | 1cm), (pH 5.4, at max 616 nm) | 530 to 570 |
| Absorptivity (1% | 1cm), (pH 3.8, at max 443 nm) | 240 to 270 |
| pH Transition Range | 3.8 (Yellow-Green) – 5.4 (Blue) |
| Loss on Drying | ≤ 3 % |
| Synonym | 3’, 3”, 5’, 5” – Tetrabromo-m-cresolsulfonephthalein; Bromocresol Green Sultone form |
