In Polymerase Chain Reaction (PCR), Brilliant Green serves as a monitoring tool for DNA amplification progress. It achieves this by binding to double-stranded DNA, aiding in the visualization of DNA bands during electrophoresis. Additionally, the binding ability of Brilliant Green to DNA prevents transcription and replication by hindering RNA polymerase from binding.
In wastewater treatment processes, the antimicrobial properties of Brilliant Green find application in inhibiting the growth of Aerobacter aerogenes, a common bacterial contaminant. This antimicrobial effect helps maintain the cleanliness and safety of treated water.
Furthermore, Brilliant Green serves a crucial role in microbial culture media preparation, acting as a pH indicator. This indicator aids in determining the acidity or alkalinity of the media, which is vital for culturing specific microorganisms. It helps microbiologists create an optimal environment for the growth of desired bacterial strains.
Overall, Brilliant Green’s multifaceted properties make it indispensable in various scientific applications. From monitoring DNA amplification to its role as an antimicrobial agent and pH indicator, Brilliant Green continues to be a valuable tool in microbiology and molecular biology labs worldwide. Its versatility and effectiveness contribute significantly to research and experimentation in the field of life sciences.
Cat. Number | ASC-1002 |
CAS Number | 633-03-4 |
MDL Number | MFCD00011880 |
Color Index | 42040 |
Pubchem | 310268543 |
Molecular Weight | 482.64 g/mol |
Molecular Formula | C27H34N2O4S |
Solubility | Soluble in water (100 mg/ml)Soluble in water (100 mg/ml) Soluble in methanol and 2-methoxyethanol (60 mg/ml) Soluble in ethanol (50 mg/ml) |
Storage Temperature / condition | 20 °C / Keep away from oxidizing agents. |
Form and Color | Powder to Crystalline Powder / Blue to Green Blue to Green |
Dye Content | ≥ 85% |
pH Transition Range | 0.0 (Yellow) - 2.6 (Green) |
Wavelength of Maximum Absorption | ~625nm in 50% ethanol |
Loss on Drying | ≤ 5 % |
Synonym | Astradiamant green GX |