Bromothymol blue sodium salt plays a vital role as a pH indicator in microbial culture media, providing a visual representation of the acidity or alkalinity of the environment. This is crucial for cultivating specific microorganisms, as their growth often depends on the optimal pH conditions.
In analytical chemistry, bromothymol blue serves as a valuable tool for detecting the presence of proteins. When the phosphatase enzyme cleaves the bonds between the dye and the protein, a blue complex is released, indicating the presence of proteins. This reaction allows scientists to detect the presence of proteins in a sample, aiding in various analytical and research applications.
Furthermore, the use of bromothymol blue in these applications demonstrates its versatility in different scientific fields. Its ability to indicate pH levels and detect proteins makes it an indispensable tool in microbiology and analytical chemistry laboratories.
Overall, bromothymol blue sodium salt is essential for monitoring pH levels in microbial cultures and detecting proteins in analytical chemistry. Its distinct properties and reactions contribute significantly to the advancement of scientific research and experimentation.
| Cat. Number | ASC-1003 |
| CAS Number | 34722-90-2 |
| MDL Number | MFCD00077263 |
| Pubchem | 310269017 |
| Molecular Weight | 614.30 gr/mol |
| Molecular Formula | C27H27Br2NaO5 |
| Storage Temperature / Condition | 20 °C / Keep away from oxidizing agents. |
| Form and Color | Powder / Green to orange to brown to black powder |
| Solution Appearance | 0.1 % in water, clear |
| Dye Content | ≥ 90 % |
| pH Transition Range | 5.8 (yellow) to 7.6 (blue) |
| Wavelength of Maximum Absorption | 615 - 618 nm |
| Absorptivity (1% / 1cm) | ≥ 450 (at λ max) |
| Loss on Drying | ≤ 5 % |
