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Bromothymol Blue Sodium Salt
pH indicator, detecting the presence of proteins
Catalog Number: ASC-1003
CAS Number: 34722-90-2
MW: 614.30 gr/mol
MF: C27H27Br2NaO5
In microbial culture media, bromothymol blue sodium salt is an essential pH indicator that gives an indication of how acidic or alkaline the surrounding solution is.
In analytical chemistry, this dye is used for detecting the presence of proteins by binding to phosphatase enzyme. A blue complex is released, signifying the presence of proteins, when the phosphatase enzyme cleaves the bonds between dye and protein, so a blue complex is released that can be seen.
| Cat. Number | ASC-1003 |
| CAS Number | 34722-90-2 |
| MDL Number | MFCD00077263 |
| Pubchem | 310269017 |
| Molecular Weight | 614.30 gr/mol |
| Molecular Formula | C27H27Br2NaO5 |
| Storage Temperature / Condition | 20 °C / Keep away from oxidizing agents. |
| Form and Color | Powder / Green to orange to brown to black powder |
| Solution Appearance | 0.1 % in water, clear |
| Dye Content | ≥ 90 % |
| pH Transition Range | 5.8 (yellow) to 7.6 (blue) |
| Wavelength of Maximum Absorption | 615 – 618 nm |
| Absorptivity (1% / 1cm) | ≥ 450 (at λ max) |
| Loss on Drying | ≤ 5 % |
