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Peptone from Casein Tryptone

For preparation of microbial culture media, especially for fastidious bacteria, molds, and bacterial vaccine production

SKU AS-1006 Category
Catalog Number: AS-1006
CAS Number: 91079-40-2

Enzymatic digestion (hydrolysis) of casein, the primary protein in milk, yields a complex combination known as tryptone. As a rich source of nutrients for bacterial growth, it is useful in microbiology laboratories.
One of the many free amino acids that can be found in tryptone is tryptophan, which is present in particularly high concentrations. This can be useful in cultivating bacteria that need tryptophan for specific metabolic processes. The lack of observable carbohydrates is another benefit of tryptone. This allows to use in culture media formulations where the presence of carbohydrates might interfere with specific test results.

Shelf life and storage
Store Peptone from Casein, Tryptone between 10-30 °C in a ventilated and low humidity place and protected from light. Close the container tightly after use. Use before expiry date.

Note that this product is for R&D use only. DO NOT USE for drug, household, or any other uses.

Typical analysis
Powder appearanceBeige, free flowing
SolubilityFreely soluble in water, not soluble in alcohol
1% solution appearanceclear
pH (10% in water)6.3 – 7.3
Chemical analysis
Total nitrogen≥11
Sodium chloride≤5
Amino nitrogen≥4
Moisture≤11
Amino acid composition (mg/g)
Aspartic acid54
Threonine32
Serine41
Glutamic acid167
Proline81
Glycine15
Alanine24
Cysteine2
Valine50
Methionine17
Isoleucine40
Leucine70
Tyrosine30
Phenylalanine39
Histidine21
Lysine60
Arginine28
Tryptophan10
Microbial Quality Control Cultural response after 18-48 hours incubation at 35-37 °C on Soybean Casein Digest Medium (AS-1370) prepared by Tryptone as a component.
StrainATCCGrowth
Escherichia coli25922Luxuriant
Salmonella typhi6539Luxuriant
Staphylococcus aureus25923Luxuriant
Enterococcus faecalis11700Luxuriant
Klebsiella pneumoniae13883Luxuriant

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