Peptone from Casein Tryptone
For preparation of microbial culture media, especially for fastidious bacteria, molds, and bacterial vaccine production
- Description
- Quality Control
Enzymatic digestion (hydrolysis) of casein, the primary protein in milk, yields a complex combination known as tryptone. As a rich source of nutrients for bacterial growth, it is useful in microbiology laboratories.
One of the many free amino acids that can be found in tryptone is tryptophan, which is present in particularly high concentrations. This can be useful in cultivating bacteria that need tryptophan for specific metabolic processes. The lack of observable carbohydrates is another benefit of tryptone. This allows to use in culture media formulations where the presence of carbohydrates might interfere with specific test results.
Shelf life and storage
Store Peptone from Casein, Tryptone between 10-30 °C in a ventilated and low humidity place and protected from light. Close the container tightly after use. Use before expiry date.
Note that this product is for R&D use only. DO NOT USE for drug, household, or any other uses.
| Powder appearance | Beige, free flowing |
| Solubility | Freely soluble in water, not soluble in alcohol |
| 1% solution appearance | clear |
| pH (10% in water) | 6.3 – 7.3 |
| Total nitrogen | ≥11 |
| Sodium chloride | ≤5 |
| Amino nitrogen | ≥4 |
| Moisture | ≤11 |
| Aspartic acid | 54 |
| Threonine | 32 |
| Serine | 41 |
| Glutamic acid | 167 |
| Proline | 81 |
| Glycine | 15 |
| Alanine | 24 |
| Cysteine | 2 |
| Valine | 50 |
| Methionine | 17 |
| Isoleucine | 40 |
| Leucine | 70 |
| Tyrosine | 30 |
| Phenylalanine | 39 |
| Histidine | 21 |
| Lysine | 60 |
| Arginine | 28 |
| Tryptophan | 10 |
| Strain | ATCC | Growth |
| Escherichia coli | 25922 | Luxuriant |
| Salmonella typhi | 6539 | Luxuriant |
| Staphylococcus aureus | 25923 | Luxuriant |
| Enterococcus faecalis | 11700 | Luxuriant |
| Klebsiella pneumoniae | 13883 | Luxuriant |










