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Hematoxylin

Hematoxylin is a natural compound that has a long history of use in biological sciences, primarily as a vital stain in histology.

Category
Catalog Number: ASC-1032
CAS Number: 517-28-2
MW: 302.29 gr/mol
MF: C16H14O6 · xH2O

Hematoxylin is a natural compound extracted from the heartwood of the logwood tree (Haematoxylum campechianum). It has a long history of use in biological sciences, primarily as a vital stain in histology. Hematoxylin specifically stains cell nuclei a deep blue or purple color, allowing detailed visualization of cellular morphology under a microscope. This staining technique, often combined with eosin (resulting in Hematoxylin and Eosin or H&E stain), is the gold standard for routine histological examination of tissues in various medical fields.

Cat. NumberASC-1032
CAS Number517-28-2
MDL NumberMFCD00078111
PubChem310274419
Molecular Weight (anhydrous)302.29 gr/mol
Molecular FormulaC16H14O6 · xH2O
Storage Temperature+20°C
Form and ColorPowder, Light-Yellow to Brown or Beige
Solubility (0.1% in ethanol)Clear to slightly turbid, yellow, orange or brown solution
Wavelength of Maximum Absorption517 – 525 nm
Absorption Ratio (λmax ± 15nm)0.9 – 1.1
Loss on Drying≤ 7%
Purity (HPLC)≥ 95%

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