Bile Salts Mixture

The bile salts mixture is used as selective inhibitor for gram positive bacteria. This product can induce germination but inhibits the outgrowth of the germinated spores. The presence of bile salts in culture media allows the growth of bile tolerant enteric microorganisms at a concentration of 0.25 – 0.5%.
Bile salts mixture is used in different culture media like MacConkey agar/broth, Violet Red Bile agar, SS agar, Hektoen enteric agar. Using Bile salts as inhibitory agent for gram-positive bacteria leads to increasing the selectivity of the media to Enterobacteriaceae.
Lactose fermenting bacteria that form acid in culture media can be detected by precipitating a very small amount of bile salts precipitate around the colony.
Using bile salts in culture media does not affect the color of pH indicator dyes in prepared media and after bacterial culture.

Shelf life and storage
Store the bile salts mixture container between 10-30 °C in a ventilated area and protected from light. Close the container tightly after use. Use before expiry date.

Note that this product is for R&D use only. DO NOT USE for drug, household, or any other uses.