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Safranin O Powder

Safranin O Powder, a common biological stain, is widely used in microbiology and histology for staining purposes.

SKU ASC-1011 Categories ,
Catalog Number: ASC-1011
CAS Number: 477-73-6
MW: 350.85 gr/mol
MF: C20H19ClN4

Common biological dye Safranin O Powder is frequently used for staining in histology and microbiology. Safranin O Powder is used by microbiologists to stain bacterial cells, making it easier to distinguish between Gram-positive and Gram-negative bacteria based on the properties of their cell walls. Gram-negative bacteria lose the stain and turn red or pink after counterstaining, but Gram-positive bacteria keep the stain and appear purple.
In histology, safranin O powder is a key staining agent, especially for identifying cartilage and cell nuclei in histological samples. This staining method allows pathologists and researchers to see cellular characteristics and analyze tissue architecture.
Safranin O powder’s application in leather and textile dyeing, which transcends the domains of histology and microbiology, demonstrates its adaptability.

Cat. NumberASC-1011
CAS Number477-73-6
MDL NumberMFCD00011759
Color Index50240
Pubchem310279833
Molecular Weight350.85 gr/mol
Molecular FormulaC20H19ClN4
Storage Temperature20 °C / Keep away from oxidizing agents.
Form and ColorCrystall / Red-brown to dark brown
Purity≥ 95 %
Solution Appearance0.1% in 50% EtOH: Red to dark red, clear
solution
SolubilitySoluble in water 50 gr/L
Soluble in alcohol
Wavelength of Maximum Absorption (λ max)530 – 534 nm (in 50% EtOH)
Specific Absorption≥ 1500 (λ max, in 50% EtOH)
Loss on Drying≤ 8 %
SynonymBasic Red2 / Cotton Red / Gossypimine / Safranin Y or A / 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride / Safranine T

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